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rat ctnt elisa kit  (Cusabio)


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    Cusabio rat ctnt elisa kit
    Rat Ctnt Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rats+ctnt/Rat+Cardiac+Troponin+%E2%85%A0(cTn-%E2%85%A0)+ELISA+kit/pm41410140-77-10-16
    Average 93 stars, based on 36 article reviews
    rat ctnt elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: The therapeutic effect of Qishen Huoxue Granule on myocardial injury in sepsis rats and its underlying mechanism via suppressing excessive autophagy
    Article Snippet: .. The serum was collected and supernatants were obtained by centrifugation at 4°C for 10 min. Then, according to the instructions of ELISA kits, ELISA detected the rats cTnT (CSB-E16443r, Cusabio, USA), BNP (CSB-E08594r, Cusabio, USA), TNF-α (BMS625, Thermo, USA), IL-1β (RTA 00, R & D, USA). ..

    Article Title: The therapeutic effect of Qishen Huoxue Granule on myocardial injury in sepsis rats and its underlying mechanism via suppressing excessive autophagy.
    Article Snippet: .. The serum was collected and supernatants were obtained by centrifugation at 4°C for 10 min. Then, according to the instructions of ELISA kits, ELISA detected the rats cTnT (CSB-E16443r, Cusabio, USA), BNP (CSB-E08594r, Cusabio, USA), TNF-α (BMS625, Thermo, USA), IL-1β (RTA 00, R & D, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The therapeutic effect of Qishen Huoxue Granule on myocardial injury in sepsis rats and its underlying mechanism via suppressing excessive autophagy
    Article Snippet: .. The serum was collected and supernatants were obtained by centrifugation at 4°C for 10 min. Then, according to the instructions of ELISA kits, ELISA detected the rats cTnT (CSB-E16443r, Cusabio, USA), BNP (CSB-E08594r, Cusabio, USA), TNF-α (BMS625, Thermo, USA), IL-1β (RTA 00, R & D, USA). ..

    Article Title: The therapeutic effect of Qishen Huoxue Granule on myocardial injury in sepsis rats and its underlying mechanism via suppressing excessive autophagy.
    Article Snippet: .. The serum was collected and supernatants were obtained by centrifugation at 4°C for 10 min. Then, according to the instructions of ELISA kits, ELISA detected the rats cTnT (CSB-E16443r, Cusabio, USA), BNP (CSB-E08594r, Cusabio, USA), TNF-α (BMS625, Thermo, USA), IL-1β (RTA 00, R & D, USA). ..



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    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker <t>cTnT</t> and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
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    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker <t>cTnT</t> and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
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    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker <t>cTnT</t> and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
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    Image Search Results


    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Journal: Frontiers in Immunology

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    doi: 10.3389/fimmu.2025.1719392

    Figure Lengend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Article Snippet: Fixed aCM in suspension were co-stained with MLC2a REAfinityTM conjugated with APC (1:10, Miltenyi Biotec) and cTnT REAfinityTM conjugated with FITC (1:10, Miltenyi Biotec) antibodies and incubated for 15 min at RT.

    Techniques: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker

    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Journal: Frontiers in Immunology

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    doi: 10.3389/fimmu.2025.1719392

    Figure Lengend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Article Snippet: Tissues were incubated with primary antibodies (cTnT REAfinity conjugated FITC (1:100, Miltenyi Biotec)) and CX3CR1 rabbit anti-human (1H14L7) (1:250, Invitrogen) or Collagen I Polyclonal Antibody (1:100, ThermoFisher Scientific) overnight at 4 °C on a plate shaker.

    Techniques: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker